SubtiBank SubtiBank
citS
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

citS

two-component sensor kinase, regulation of citrate uptake
locus
BSU_07580
pI
6.48
mw
59.72 kDa
protein length
542 aa Sequence Blast
gene length
1629 bp Sequence Blast
function
regulation of citrate uptake
product
two-component sensor kinase
essential
no
synonyms
yflR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    830,945 832,573

    Phenotypes of a mutant

  • no growth with citrate as sole carbon source [Pubmed|10972810]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • [SW|Histidine kinase domain] (aa 336-528) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity may be controlled by interaction with [protein|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|YflP] [Pubmed|14499931]

    • [SW|Localization]

  • cell membrane (Heterogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]-[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]-[gene|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|yflP]
    description
    [Pubmed|10972810]
    view in new tab

    Biological materials

    Mutant

  • MGNA-C333 (citS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2331 NBRP B. subtilis, Japan]
  • BKE07580 ([gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
  • BKK07580 ([gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA

    • References

    Reviews

  • 28152228

    • Original publications

  • 10094672,16842348,10972810,16479537,14499931