ybaF

trans-membrane T component of [SW|ECF transporter]s

locus: BSU_01470

pI: 10.18

mw: 29.54 kDa

protein length: 265 aa

gene length: 798 bp

function: uptake of micronutrients

product: [SW|ECF transporter] (membrane-spanning protein)

essential: no

synonyms: ecfT

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.1|The general components of the ECF transporters]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 152,130 152,927

The protein

Protein family

• energy-coupling factor EcfT family (single member, according to UniProt)

Structure

• [PDB|4HUQ] (EcfA1-EcfA2-EcfT-FolT complex of ''Lactobacillus brevis''), [Pubmed|23584589]

[SW|Localization]

• cell membrane [Pubmed|20497229]

Biological materials

Mutant

• MGNA-B945 (ybaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1944 NBRP B. subtilis, Japan]
• BKE01470 ([gene|7DC7BD54ED425859FFD45B8B6F3522750A938835|ybaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTGCCGATAATCATGCTGT, downstream forward: _UP4_TTCTTAAGGGCTTAGAGGTG
• BKK01470 ([gene|7DC7BD54ED425859FFD45B8B6F3522750A938835|ybaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTGCCGATAATCATGCTGT, downstream forward: _UP4_TTCTTAAGGGCTTAGAGGTG
• BP1149 ([gene|D8637043C7E2291589C966E95F94DC61B1A8AB75|ybxA]-[gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ybaE]-[gene|7DC7BD54ED425859FFD45B8B6F3522750A938835|ybaF]::cat trp⁺) available at [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

References

• 10092453,20497229,22574898,24362466