SubtiBank SubtiBank
pncB
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

pncB

putative nicotinate phosphoribosyltransferase
locus
BSU31750
pI
5.09
mw
56.01 kDa
protein length
490 aa Sequence Blast
gene length
1470 bp Sequence Blast
function
unknown
product
putative nicotinate phosphoribosyltransferase
essential
yes
ec
2.4.2.11
synonyms
yueK

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    3,259,403 → 3,260,875

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family

  • NAPRTase family (according to Swiss-Prot)
  • Structure

  • [PDB|2F7F] (from ''Enterococcus faecalis'', 61% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]
    description
    [Pubmed|9274030]
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    Biological materials

    Mutant

  • MGNA-B562 (yueK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1561 NBRP B. subtilis, Japan]
  • References

  • 16199587,9274030