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spoIIE [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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spoIIE [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA
locus
BSU00640
pI
6.00
mw
91.00 kDa
function
control of SigF activity
required for normal formation of the asymmetric septum
product
protein serine phosphatase, septum-associated PP2C
essential
no
ec
3.1.3.16
synonyms
spoIIH, spoIIK

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|sigma factors and their control], [SW|membrane proteins], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    70,538 -> 73,021

    The protein

    Catalyzed reaction/ biological activity

  • dephosphorylation of [SW|SpoIIAA]
  • Protein family

  • PP2C [SW|phosphatase]
  • Effectors of protein activity

  • degraded by [SW|FtsH], oligomerization and polar localization protect SpoIIE from degradation [Pubmed|26465112]
  • Structure

  • [PDB|3T91] (phosphatase domain) [Pubmed|22115775]
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • localizes to the polar cell division sites where it causes [protein|search|FtsZ] to relocate from mid-cell to form polar Z-rings
  • forespore [Pubmed|26465112]
  • localizes to the cell poles (via [SW|DivIVA]) [Pubmed|26465112]
  • proper localization and stability depend on [SW|SpoIIQ] [Pubmed|26929302]
  • [SW|Interactions]

  • [SW|SpoIIE]-[protein|search|FtsZ]
  • [SW|SpoIIE]-[SW|SpoIIAB]
  • [SW|SpoIIE]-[SW|SpoIIAA] [Pubmed|11846550]
  • [SW|SpoIIE]-[SW|SpoIIE], forms a dimer [Pubmed|22115775]
  • [SW|SpoIIQ]-[SW|SpoIIE]
  • [SW|DivIVA]-[SW|SpoIIE], this anchors SpoIIE to the cell poles [Pubmed|25101664]
  • [SW|SpoIIE]-[SW|RodZ] [Pubmed|27415800]
  • [SW|SpoIIE]-[SW|FtsH] [Pubmed|26465112]
  • Expression and Regulation

    Operon

  • ''spoIIE'' (DBTBS)
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1556084]
  • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
  • Labs working on this gene/protein

  • [SW|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
  • References

    Modeling of [protein|search|SigF] activation

  • 24067622,22312331
  • Original Publications

  • 27415800,7570023,7570022,12180929,9077448,9364910,10323866,8622920,12007411,16824103,10476035,10747015,10322028,15978076,9495766,14744853,9642177,15866939,8846916,1556084,1948031,9573053,18077456,11846550,9150232,15205443,1556084,15687200,19578359,10049355,20817757,22115775,25101664,11846550,26465112,26929302