SubtiBank SubtiBank
spoIIE [2017-03-30 15:17:27]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

spoIIE [2017-03-30 15:17:27]

protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA
locus
BSU00640
pI
6.00
mw
91.00 kDa
protein length
827 aa Sequence Blast
gene length
2481 bp Sequence Blast
function
control of SigF activity
required for normal formation of the asymmetric septum
product
protein serine phosphatase, septum-associated PP2C
essential
no
ec
3.1.3.16
synonyms
spoIIH, spoIIK

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]
  • Gene

    Coordinates
    70,538 -> 73,021

    The protein

    Catalyzed reaction/ biological activity

  • dephosphorylation of [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA]
  • Protein family

  • PP2C [SW|phosphatase]
  • Effectors of protein activity

  • degraded by [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH], oligomerization and polar localization protect SpoIIE from degradation [Pubmed|26465112]
  • Structure

  • [PDB|3T91] (phosphatase domain) [Pubmed|22115775]
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • localizes to the polar cell division sites where it causes [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] to relocate from mid-cell to form polar Z-rings
  • forespore [Pubmed|26465112]
  • localizes to the cell poles (via [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA]) [Pubmed|26465112]
  • proper localization and stability depend on [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] [Pubmed|26929302]
  • Expression and Regulation

    Operons

    genes
    [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]
    description
    (DBTBS) null

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1556084], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|1556084,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
  • view in new tab

    genes
    [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-A083 (spoIIE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/83 NBRP B. subtilis, Japan]
  • Labs working on this gene/protein

  • [SW|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
  • References

    Modeling of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activation

  • 24067622,22312331
  • Original Publications

  • 27415800,7570023,7570022,12180929,9077448,9364910,10323866,8622920,12007411,16824103,10476035,10747015,10322028,15978076,9495766,14744853,9642177,15866939,8846916,1556084,1948031,9573053,18077456,11846550,9150232,15205443,1556084,15687200,19578359,10049355,20817757,22115775,25101664,11846550,26465112,26929302