spoIIE [2017-03-31 09:36:17]

protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA

locus: BSU00640

pI: 6.00

mw: 91.00 kDa

protein length: 827 aa

gene length: 2481 bp

function: control of SigF activity
required for normal formation of the asymmetric septum

product: protein serine phosphatase, septum-associated PP2C

essential: no

ec: 3.1.3.16

synonyms: spoIIH,spoIIK

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|Spo0A regulon]

Gene

Coordinates: 70,538 -> 73,021

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA]

Protein family

• PP2C [SW|phosphatase]

[SW|Cofactors]

• Mn ²⁺ [pubmed|28358838]

Effectors of protein activity

• degraded by [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH], oligomerization and polar localization protect SpoIIE from degradation [Pubmed|26465112]

Structure

• [PDB|3T91] (phosphatase domain) [Pubmed|22115775]

[SW|Localization]

• cell membrane (according to Swiss-Prot)
• localizes to the polar cell division sites where it causes [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] to relocate from mid-cell to form polar Z-rings
• forespore [Pubmed|26465112]
• localizes to the cell poles (via [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA]) [Pubmed|26465112]
• proper localization and stability depend on [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] [Pubmed|26929302]

Expression and Regulation

Operons

• genes: [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]
  description: (DBTBS) null

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1556084], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|1556084,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]

• genes: [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
  description: [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325]

Biological materials

Mutant

• MGNA-A083 (spoIIE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/83 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

References

Modeling of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activation

• 24067622,22312331

Original Publications

• 27415800,7570023,7570022,12180929,9077448,9364910,10323866,8622920,12007411,16824103,10476035,10747015,10322028,15978076,9495766,14744853,9642177,15866939,8846916,1556084,1948031,9573053,18077456,11846550,9150232,15205443,1556084,15687200,19578359,10049355,20817757,22115775,25101664,11846550,26465112,26929302,28358838