yneA

inhibits cell division during SOS response

locus: BSU_17860

pI: 4.61

mw: 11.75 kDa

protein length: 105 aa

gene length: 318 bp

function: inhibits cell division during SOS response

product: checkpoint enforcement protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]

Gene

Coordinates: 1,918,406 1,918,723

Phenotypes of a mutant

• increased sensitivity to DNA-damaging agents [Pubmed|23728628]
• inactivation allows growth of a [gene|search|whiA ][gene|search|parB ]double mutant [pubmed|29378890]

The protein

Protein family

• YneA family (single member, according to UniProt)

[SW|Domains]

• contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] (aa 38-89) [Pubmed|20400548,18430080]

[SW|Localization]

• secreted to the medium (signal peptide) [Pubmed|20400548]

Expression and Regulation

Operons

• genes: [gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]-[gene|67074435B8FDA85C8ED4D8C4ECE3580C4EDFBF2A|yneB]-[gene|5BFD552F8A467C8D95FABE87CC8C5E7F04114886|ynzC]
  description: [Pubmed|12581363]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|12581363], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|12581363]

  additional information

  • YneA is rapidly degraded by extracellular proteases ([protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA]) [PubMed|29979679,20400548]

Biological materials

Mutant

• MGNA-B388 (yneA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1387 NBRP B. subtilis, Japan]
• BKE17860 ([gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACCTCCAACAGGAATG, downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA
• BKK17860 ([gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACCTCCAACAGGAATG, downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA

References

Reviews

• 22933559,18430080

Original publications

• 12581363,20400548,16267290,23728628,29378890,16549676,29979679,30315724