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mcsB [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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mcsB [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

protein arginine kinase, tags proteins for degradation by ClpCP, adaptor protein, modulator of CtsR-dependent repression
locus
BSU00850
pI
5.00
mw
40.00 kDa
function
control of CtsR activity
product
protein arginine kinase
essential
no
synonyms
yacI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|transcription factors and their control], [SW|sporulation proteins], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon], [SW|SigF regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    102,484 -> 103,575

    The protein

    Catalyzed reaction/ biological activity

  • phosphorylates and thereby targets non-functional CtsR for degradation by [protein|search|ClpP]/[protein|search|ClpC] [Pubmed|20852588]

    • Protein family

  • ATP:guanido phosphotransferase family (according to Swiss-Prot)

    • Modification

  • autophosphorylation on specific arginine residues [Pubmed|20852588,21622759], dephosphorylation by [protein|search|YwlE] [Pubmed|20852588,21622759]
  • autophosphorylation stimulates McsB activity as adaptor protein for [protein|search|ClpC] [Pubmed|21622759]

    • [SW|Interactions]

  • [protein|search|McsA]-[protein|search|McsB]-[protein|search|CtsR], [protein|search|McsB]-[protein|search|CtsR] [Pubmed|20852588], [protein|search|McsA]-[protein|search|McsB]
  • [protein|search|McsB]-[protein|search|YwlE] [Pubmed|20852588]
  • [protein|search|McsB]-[protein|search|ClpP]/[protein|search|ClpC] (rapid degradation of phosphorylated McsB) [Pubmed|20852588]

    • Expression and Regulation

    Operon

  • ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [protein|search|SigF] [Pubmed|16497325]

    • Regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]
  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]
  • induced by heat ([protein|search|CtsR]) [Pubmed|9987115]

    • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9987115]

    • Biological materials

    Mutant

  • ''mcsB::aphA3'' availbale from the Gerth lab
  • mcsBC167S::spec available from the Gerth lab
  • GP1457 (''mcsB''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BP69 (spc), available in [SW|Fabian Commichau]'s lab

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • expression of native ''mcsB'' in ''B. subtilis'': pBP186 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

    • Antibody

  • available in Gerth lab

    • Labs working on this gene/protein

  • [SW|Ulf Gerth], Greifswald, Germany
  • [SW|Fabian Commichau] Göttingen, Germany

    • References

    Reviews

  • 23375660,19609260

    • Original Publications

  • 17380125,16163393,19498169,11179229,9987115,8793870,16497325,19226326,9987115,11544224,20852588,21622759,22517742,24825175,24263382,25610436,26458230,27749819