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mcsB [2019-08-15 13:03:39]
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mcsB [2019-08-15 13:03:39]

protein arginine kinase, tags proteins for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP], adaptor protein, modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]-dependent repression
locus
BSU_00850
pI
5.07
mw
40.97 kDa
protein length
363 aa Sequence Blast
gene length
1092 bp Sequence Blast
function
control of [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR] activity
product
protein arginine kinase
essential
no
synonyms
yacI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • Gene

    Coordinates
    102,484 103,575

    Phenotypes of a mutant

  • more rapid spore germination as compared to wild type cells [pubmed|31221751]

    • The protein

    Catalyzed reaction/ biological activity

  • phosphorylates and thereby targets non-functional [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR] for degradation by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]/[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] [Pubmed|20852588]
  • ATP + L-arginyl-[protein] --> ADP + H+ + Nω-phospho-L-arginyl-[protein] (according to UniProt)

    • Protein family

  • ATP:guanido phosphotransferase family (single member, according to UniProt)

    • Modification

  • autophosphorylation on specific arginine residues [Pubmed|20852588,21622759], dephosphorylation by [protein|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|YwlE] [Pubmed|20852588,21622759]
  • autophosphorylation stimulates [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] activity as adaptor protein for [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] [Pubmed|21622759]

    • Structure

  • [PDB|6FH1] [pubmed|30962626]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • Biological materials

    Mutant

  • MGNA-B930 (yacI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1929 NBRP B. subtilis, Japan]
  • ''mcsB::aphA3'' availbale from the Gerth lab
  • mcsBC167S::spec available from the Gerth lab
  • GP1457 (''mcsB''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BP69 (spc), available in [SW|Fabian Commichau]'s lab
  • BKE00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC
  • BKK00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC

    • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • expression of native ''mcsB'' in ''B. subtilis'': pBP186 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

    • Antibody

  • available in Gerth lab

    • labs

  • [SW|Ulf Gerth], Greifswald, Germany
  • [SW|Fabian Commichau] Göttingen, Germany [SW|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

    • References

    Reviews

  • 23375660,19609260,28748186

    • Original Publications

  • 17380125,16163393,19498169,11179229,9987115,8793870,16497325,19226326,9987115,11544224,20852588,21622759,22517742,24825175,24263382,25610436,26458230,27749819,30962626,31221751