lcfA

long chain acyl-CoA synthetase, involved in surfactin production

locus: BSU_28560

pI: 6.12

mw: 62.52 kDa

protein length: 560 aa

gene length: 1683 bp

function: fatty acid degradation

product: long chain acyl-CoA synthetase

essential: no

ec: 6.2.1.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 2,918,646 2,920,328

The protein

Catalyzed reaction/ biological activity

• long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
• activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

Protein family

• [SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Paralogous protein(s)

• [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|LcfB], [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|YhfT], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|YngI]

Structure

• [PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) [pubmed|22560731]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]-[gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21398533], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|21398533], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by long chain acyl-CoA (C14 ... C20) ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
• 1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
• BKE28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
• BKK28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT

References

• 17189250,17919287,20797616,17085570,22900538,22560731,31113899