SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

yfkT

general stress protein, part of the [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|YfkQ]-[protein|B7EEC1910715507596734540D24B3C93B4B7065F|YfkR]-[protein|7A62875A659B9F0967C392F38895D9E15A29D559|YfkT] germinant receptor of unknown specificity

locus: BSU_07760

pI: 9.77

mw: 40.20 kDa

protein length: 358 aa

gene length: 1077 bp

function: germination

product: part of the [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|YfkQ]-[protein|B7EEC1910715507596734540D24B3C93B4B7065F|YfkR]-[protein|7A62875A659B9F0967C392F38895D9E15A29D559|YfkT] germinant receptor

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.5|Germination/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 846,182 847,258

The protein

Protein family

• [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
• [SW|Spore germination protein (SGP) (TC 2.A.3.9) family] (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]-[gene|B7EEC1910715507596734540D24B3C93B4B7065F|yfkR]-[gene|AA76BEF6A4CECB5D3910918E8D91D8B919EDEA7E|yfkS]-[gene|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • expressed during [SW|sporulation] in the forespore [Pubmed|15699190]

Biological materials

Mutant

• MGNA-C343 (yfkT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2341 NBRP B. subtilis, Japan]
• BKE07760 ([gene|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAGCACATCCTTTCTG, downstream forward: _UP4_TTGTTCGGCGCAAAAGCAAA
• BKK07760 ([gene|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAGCACATCCTTTCTG, downstream forward: _UP4_TTGTTCGGCGCAAAAGCAAA

References

• 15805528,15699190,22383849,10762253,27766092