htrB

membrane-anchored protein quality control protease, serine protease, response to secretion and heat stresses

locus: BSU_33000

pI: 4.63

mw: 48.55 kDa

protein length: 458 aa

gene length: 1377 bp

function: protein quality control

product: serine protease

essential: no

ec: 3.4.21.107

synonyms: yvtA,yvtB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.1|Protein quality control]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,384,070 3,385,446

The protein

Catalyzed reaction/ biological activity

• Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val (according to UniProt)

Protein family

• Peptidase S1C family (with [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|HtrA] and [protein|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|HtrC], according to UniProt)

[SW|Domains]

• cytoplasmic domain (aa 1 - 71) (according to UniProt)
• transmembrane helix (aa 72 - 92) (according to UniProt)
• extracellular domain (aa 93 - 458) (according to UniProt)
• [SW|PDZ domain] (aa 350- 449) (according to UniProt)

Structure

• [PDB|3QO6] (from ''Arabidopsis thaliana'', 38% identity) [Pubmed|21532594]

[SW|Localization]

• cell membrane (according to UniProt)
• randomly distributed in foci throughout the cell surface [Pubmed|22307758]

Expression and Regulation

Operons

• genes: [gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR]: activation, [Pubmed|12270824], in [regulon|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR regulon]

  regulation

  • expressed under conditions of secretion stress ([protein|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR]) [Pubmed|12270824]

  additional information

  • [protein|796216BE9CD6DADFEADC29497253C81BF496A2ED|HtrB] is subject to degradation by [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|WprA] and other extracellular proteases [PubMed|24362423]

Biological materials

Mutant

• MGNA-B207 (yvtA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1206 NBRP B. subtilis, Japan]
• BKE33000 ([gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
• BKK33000 ([gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG

References

Reviews

• 22688815,21326199,25212246,31001739

Original publications

• 17600057,22307758,12270824,22092710,24362423,24115457,11133960,21532594,32111210