D-alanyl-D-alanine carrier protein ligase, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides
function
biosynthesis of teichoic acid
product
D-alanyl-D-alanine carrier protein ligase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]Gene
Coordinates
3,952,275 → 3,953,786
Phenotypes of a mutant
increased sensitivity to lysozyme [Pubmed|21856855]more sensitive to nisin [Pubmed|23980836]The protein
Catalyzed reaction/ biological activity
ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) (according to Swiss-Prot)Protein family
DltA subfamily (according to Swiss-Prot)Structure
[PDB|3E7X][SW|Localization]
cytoplasm (according to Swiss-Prot)Biological materials
Mutant
BKE38500 (Δ[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC, downstream forward: _UP4_CGCATTGGCGAAGAGGTTCTBKK38500 (Δ[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC, downstream forward: _UP4_CGCATTGGCGAAGAGGTTCTLabs working on this gene/protein
[SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]References
Reviews
Original publications
14762009,15955059,18784082,23980836,7797557,19324056,16306698,21856855,21926231