SubtiBank SubtiBank
rpsD
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

rpsD

ribosomal protein
locus
BSU29660
pI
10.24
mw
22.69 kDa
protein length
200 aa Sequence Blast
gene length
600 bp Sequence Blast
function
translation
product
ribosomal protein S4 (BS4)
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    3,035,730 → 3,036,332

    Phenotypes of a mutant

  • essential [Pubmed|23002217,12682299]
  • The protein

    Protein family

  • [SW|ribosomal protein] S4P family (according to Swiss-Prot)
  • Structure

  • [PDB|1C06] (Geobacillus stearothermophilus)
  • [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]
  • Expression and Regulation

    Operons

    genes
    [gene|789D08564463542B8D96FC12F408549A669703CE|rpsD]
    description
    [Pubmed|1699930]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1699930], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • autorepression of ''rpsD'' expression upon binding of excess [protein|search|RpsD] to a [SW|RNA switch] in the untranslated region of the ''rpsD'' mRNA [Pubmed|23611891,1906866]
  • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''rpsD'' [Pubmed|27120414]
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    References

  • 1699930,2121712,6420647,1400226,1906866,19653700,11489846,23611891,23002217,25548247,25548248,26115952,25903689,27120414