comEA

membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake

Locus: BSU_25590

Isoelectric point: 4.82

Molecular weight: 21.62 kDa

Protein length: 205 aa

Gene length: 618 bp

Function: genetic competence

Product: membrane DNA receptor

Essential: no

Synonyms: comD

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → Genetic competence
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,640,513 2,641,130

Phenotypes of a mutant

• strongly reduced transformation rate PubMed

The protein

Domains

• extracellular DNA-binding domain
• 2 HhH domains (aa 142-171,aa 172-302) (according to UniProt)

Structure

• 2DUY (from Thermus thermophilus hb8, 37% identity, 60% similarity)

Localization

• cell membrane, no distinct position PubMed
• integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: comEA-comEB-comEC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

Other regulations

• ComN: post-translation control,

Biological materials

Mutant

• BKE25590 (comEA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT, downstream forward: _UP4_TGATTTGACGGTCATGCATT
• BKK25590 (comEA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT, downstream forward: _UP4_TGATTTGACGGTCATGCATT

References

Reviews

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Original publications

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