mntB

manganese [SW|ABC transporter] (ATP-binding protein)

locus: BSU_30760

pI: 9.41

mw: 27.73 kDa

protein length: 250 aa

gene length: 753 bp

function: manganese uptake

product: manganese [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms: ytgB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 3,144,267 3,145,019

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• [SW|ABC transporter domain] (aa 4-236) (according to UniProt)

Structure

• [PDB|2NQ2] (from Haemophilus influenzae, 32% identity) [pubmed|17158291]

[SW|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A286 (ytgB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/286 NBRP B. subtilis, Japan]
• BKE30760 ([gene|7821C82C6C30079F713B84F1E4D654E07CE5072E|mntB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAACATATCCTCCACCTCTT, downstream forward: _UP4_GAAGGACATAAGGAGTGAGC
• BKK30760 ([gene|7821C82C6C30079F713B84F1E4D654E07CE5072E|mntB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAACATATCCTCCACCTCTT, downstream forward: _UP4_GAAGGACATAAGGAGTGAGC

labs

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

• 10760146,10092453,12950915,10760146,10760146,17158291