yhcB [2019-09-05 12:36:31]

similar to oxidoreductase

locus: BSU_09020

pI: 4.64

mw: 18.87 kDa

protein length: 176 aa

gene length: 531 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 979,396 979,926

The protein

Structure

• [PDB|4LA4] (from Pseudomonas sp., 34% identity)

Expression and Regulation

Operons

• genes: [gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]-[gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]-[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]-[gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]-[gene|95EED3A91483502CA78067421F678B1EDB965B18|yhcC]
  description: [Pubmed|22383849]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 4 to 41 min) [PubMed|21815947]

Biological materials

Mutant

• MGNA-B472 (yhcB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1471 NBRP B. subtilis, Japan]
• BKE09020 ([gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGGAAACCTCCCTGT, downstream forward: _UP4_GCAGGACAGTAAGGAGAGGT
• BKK09020 ([gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGGAAACCTCCCTGT, downstream forward: _UP4_GCAGGACAGTAAGGAGAGGT

References

• 21815947