htrC

similar to quality control membrane serine protease [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|HtrA]

locus: BSU_40360

pI: 5.32

mw: 42.63 kDa

protein length: 400 aa

gene length: 1203 bp

function: unknown

product: putative quality control membrane protease

essential: no

synonyms: yycK,yyxA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.1|Protein quality control]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,147,567 4,148,769

The protein

Protein family

• Peptidase S1C family (with [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|HtrA] and [protein|796216BE9CD6DADFEADC29497253C81BF496A2ED|HtrB], according to UniProt)

Paralogous protein(s)

• [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|HtrA]

[SW|Domains]

• transmembrane helix (aa 22 - 42) (according to UniProt)
• [SW|PDZ domain] (aa 295 - 392) (according to UniProt)

Structure

• [PDB|3QO6] (from Arabidopsis thaliana, 37% identity) [pubmed|21532594]

[SW|Localization]

• inner spore membrane [Pubmed|26731423]

Expression and Regulation

Operons

• genes: [gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
  description: [Pubmed|9829949]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|9829949], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]

• genes: [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
  description: [Pubmed|9829949]

  regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]

Biological materials

Mutant

• MGNA-B823 (yycK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1822 NBRP B. subtilis, Japan]
• BKE40360 ([gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCCATCCTTTCCAAG, downstream forward: _UP4_TAATAAAAGCAGTCTGGCAT
• BKK40360 ([gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCCATCCTTTCCAAG, downstream forward: _UP4_TAATAAAAGCAGTCTGGCAT

References

Reviews

• 21326199

Original publications

• 17600057,12270824,11555295,18957862,10692364,21624103,22307758,22092710,24362423,9829949,21630458,24115457,25384476,26731423,21532594