hemN

anaerobic coproporphyrinogen III oxidase

locus: BSU_25500

pI: 5.53

mw: 41.40 kDa

protein length: 379 aa

gene length: 1140 bp

function: heme biosynthesis

product: anaerobic coproporphyrinogen III oxidase

essential: no

synonyms: yqeR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]

Gene

Coordinates: 2,629,718 2,630,857

The protein

Protein family

• anaerobic coproporphyrinogen-III oxidase family (with [protein|5A1E474D2A6043ACF0DF00FEA7F66BFB1332C691|HemZ], according to UniProt)

Paralogous protein(s)

• [protein|5A1E474D2A6043ACF0DF00FEA7F66BFB1332C691|HemZ]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]
• SAM (according to UniProt)

Structure

• [PDB|1OLT] (from E. coli, 26% identity) [pubmed|14633981]

Expression and Regulation

Operons

• genes: [gene|3744083E3BB30A64D15360A12636665F76C40327|lepA]-[gene|757C51296E198849EAC1FFA4D37121D9085930A8|hemN]
  description: [Pubmed|8757728]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9371469], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  additional information

  • There is a terminator between '[protein|search|lepA]' and '[protein|search|hemN]' with only little readthrough

Biological materials

Mutant

• BKE25500 ([gene|757C51296E198849EAC1FFA4D37121D9085930A8|hemN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCACCTTCTTTA, downstream forward: _UP4_TAATTGACATTTTTCTTGTG
• BKK25500 ([gene|757C51296E198849EAC1FFA4D37121D9085930A8|hemN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCACCTTCTTTA, downstream forward: _UP4_TAATTGACATTTTTCTTGTG

References

• 9371469,8757728,10498703,14633981