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yugO

potassium channel protein (exporter)

locus: BSU_31322

pI: 7.00

mw: 37.00 kDa

protein length: 328 aa

gene length: 987 bp

function: potassium exporter

product: potassium channel protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,218,854 3,219,840

Phenotypes of a mutant

• reduced [SW|biofilm formation] (can be suppressed by inactivation of ''[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]'') [Pubmed|23737939]

The protein

[SW|Domains]

• contains a [SW|RCK_N domain] (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])

Structure

• [PDB|3RBZ] (from Methanothermobacter thermautotrophicus, 24% identity)

[SW|Localization]

• inner spore membrane [Pubmed|26731423]

Additional information

• potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain [Pubmed|26503040]

Expression and Regulation

Operons

• genes: [gene|C5C72925567927CC177CCAC5369F80D57B9FAB5F|mstX]-[gene|752860E73AD1A72D6598F995B80F5B21A77A5CBD|yugO]
  description: [Pubmed|23737939]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|23737939], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

  regulation

  • repressed by [protein|search|SinR] [Pubmed|23737939]

  additional information

  • potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain [Pubmed|26503040]

Biological materials

Mutant

• MGNA-A624 (yugO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/624 NBRP B. subtilis, Japan]
• GP2098 (''yugO''::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|28679749]
• GP2296 (''yugO''::''tet''), available in [SW|Jörg Stülke]'s lab
• BKE31322 ([gene|752860E73AD1A72D6598F995B80F5B21A77A5CBD|yugO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31322 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGAAATAAATATCCGATTTG, downstream forward: _UP4_GAAACAGATCAATTCCTTGC
• BKK31322 ([gene|752860E73AD1A72D6598F995B80F5B21A77A5CBD|yugO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31322 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGAAATAAATATCCGATTTG, downstream forward: _UP4_GAAACAGATCAATTCCTTGC

FLAG-tag construct

• GP2442 ''yugO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 26503058,27935846,28086088,28622518,31361596

Original publications

• 23737939,26503040,26731423,10617203,28086091,28386026