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ytxM [2019-09-20 12:10:12]
similar to prolyl aminopeptidase
function
ubiquinone and menaquinone biosynthesis
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.9|Biosynthesis of menaquinone/ based on similarity]Gene
Coordinates
3,149,751 3,150,575
The protein
Catalyzed reaction/ biological activity
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate --> (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate (according to UniProt)Protein family
[SW|AB hydrolase superfamily] (according to UniProt)Structure
[PDB|2XMZ] (from ''Staphylococcus aureus'', 34% identity) [Pubmed|21513522]Biological materials
Mutant
MGNA-A283 (ytxM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/283 NBRP B. subtilis, Japan]BKE30810 ([gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACACCATCTGATACCGTTA, downstream forward: _UP4_TGACTCATTCACATAGAGAABKK30810 ([gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACACCATCTGATACCGTTA, downstream forward: _UP4_TGACTCATTCACATAGAGAAReferences
10913081,9387221,1629163,8550504,12454479,21513522