cotA

laccase, bilirubin oxidase, spore coat protein (outer)

locus: BSU_06300

pI: 5.89

mw: 58.33 kDa

protein length: 513 aa

gene length: 1542 bp

function: resistance of the spore

product: laccase, bilirubin oxidase

essential: no

ec: 1.3.3.5

synonyms: pig

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]
• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]

Gene

Coordinates: 683,462 685,003

The protein

Protein family

• S.antibioticus phenoxazinone synthase (PhsA)

Structure

• [PDB|2BHF] (reduced form)

[SW|Localization]

• outer spore coat, more abundant at the mother cell-distal pole of the forespore, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|CotE] [Pubmed|22171814,19933362]

Expression and Regulation

Operons

• genes: [gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,3135411], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836]

  additional information

  • [protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• 1S101 ( ''cotA''::''cat''), [Pubmed|2821284], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S101&Search=1S101 BGSC]
• 1S134 ( ''cotA''::''kan''), [Pubmed|11514528], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S134&Search=1S134 BGSC]
• BKE06300 ([gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC
• BKK06300 ([gene|73473E6556D374C8623A426E075038AD01AB7025|cotA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC

References

Reviews

• 23202530

Original publications

• 15699190,3135411,11514528,1518043,2821284,19933362,20200715,20551082,20822511,21369750,22281748,23859715,22171814,22410485,15383836,26062535,24293734,24733162,25259857,25847445,26784443,27050268,28870294,29301022