SubtiBank SubtiBank
yflS
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yflS

malate transporter
locus
BSU_07570
pI
9.74
mw
51.27 kDa
protein length
478 aa Sequence Blast
gene length
1437 bp Sequence Blast
function
malate uptake
product
malate transporter
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    829,382 830,818

    RNA

    additional information

  • the mRNA is stabilized by basepairing with the RoxS RNA [pubmed|28436820]
  • non-protected mRNA is subject to degradation by by [SW|rnjA|RNase J1] [pubmed|28436820]
  • The protein

    Catalyzed reaction/ biological activity

  • uptake of malate
  • Protein family

  • SLC13A/DASS transporter (TC 2.A.47) family (single member, according to UniProt)
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • spore membrane [pubmed|30602489]
  • Expression and Regulation

    Operons

    genes
    [gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]
    description
    [Pubmed|21815947]

    regulatory mechanism

  • [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]
  • regulation

  • induced by malate ([protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR])
  • additional information

  • the mRNA is substantially stabilized upon depletion of [SW|Rny|RNase Y ][PubMed|21815947]
  • the mRNA is stabilized by basepairing with the [protein|search|RoxS ]RNA [pubmed|28436820]
  • non-protected mRNA is subject to degradation by by [SW|rnjA|RNase J1] [pubmed|28436820]
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    Biological materials

    Mutant

  • MGNA-C249 (yflS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2247 NBRP B. subtilis, Japan]
  • GP1450 (cat), available in [SW|Jörg Stülke]'s lab
  • BKE07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
  • BKK07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
  • References

  • 12949159,21815947,28436820,29651979,30602489