mhqA

hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone

locus: BSU_12870

pI: 5.40

mw: 35.23 kDa

protein length: 316 aa

gene length: 951 bp

function: resistence to methyl-hydroxyquinone

product: hydroquinone-specific dioxygenase

essential: no

synonyms: ykcA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 1,353,080 1,354,030

The protein

Protein family

• [SW|extradiol ring-cleavage dioxygenase family] (according to UniProt)

Paralogous protein(s)

• [protein|A1CB3DCF2EC78B9B0585CA6AC6F5BB37B8AF0208|MhqE], [protein|ACAFB2072E74CC059B492A31EF492028B96C569F|MhqO]

[SW|Domains]

• 2 [SW|VOC domain]s (aa 5-131, aa 154-278) (according to UniProt)

Structure

• [PDB|3OAJ] ([protein|ACAFB2072E74CC059B492A31EF492028B96C569F|MhqO], 34% identical residues)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-A737 (ykcA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/737 NBRP B. subtilis, Japan]
• BKE12870 ([gene|7334017333600B876030056BE9247AD4E8996A1D|mhqA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCTGTCCCTCCAGGT, downstream forward: _UP4_TAAATTGTGATTCTTTTGCC
• BKK12870 ([gene|7334017333600B876030056BE9247AD4E8996A1D|mhqA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCTGTCCCTCCAGGT, downstream forward: _UP4_TAAATTGTGATTCTTTTGCC

References

• 17407181,17725564,27965289