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mneS
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mneS

secondary manganese (II) efflux pump
locus
BSU_06320
pI
5.63
mw
31.63 kDa
protein length
290 aa Sequence Blast
gene length
873 bp Sequence Blast
function
Mn(II) export
product
manganese (II) efflux pump
essential
no
synonyms
undefined,ydxT,yeaB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.11|Other exporters]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.13|Metal ion exporters]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    686,962 687,834

    Phenotypes of a mutant

  • a [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] [protein|72BF9B934CB5800E156263FF377DED0AD2E920A9|MneS] double mutant is extremely senitive to Mn2+ intoxication [pubmed|31685536]

    • The protein

    Catalyzed reaction/ biological activity

  • efflux of Mn(II) [Pubmed|27748968]

    • Protein family

  • [SW|Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family] [Pubmed|27748968]

    • Paralogous protein(s)

  • [protein|D52111E81E9233DABAEFCAF6217145CF6A7F3961|YdbO], [protein|28C7CFE5701F6920652BE1973DC7A15D7C088E35|MneP]

    • Structure

  • [PDB|5VRF] (from Shewanella oneidensis, 28% identity) [pubmed|29507252]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|27748968], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]: transcription activation, [Pubmed|27748968], in [regulon|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR regulon]
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    Biological materials

    Mutant

  • MGNA-A914 (yeaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/914 NBRP B. subtilis, Japan]
  • BKE06320 ([gene|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAAACCTCCTCTCGC, downstream forward: _UP4_TAATGGAGCTTCCTGTCACA
  • BKK06320 ([gene|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAAACCTCCTCTCGC, downstream forward: _UP4_TAATGGAGCTTCCTGTCACA

    • References

  • 27748968,29507252,31685536