copA [2017-8-11 16:0:3]

copper-transporting ATPase, resistance to copper

locus: BSU33500

pI: 5.48

mw: 85.83 kDa

protein length: 803 aa

gene length: 2409 bp

function: copper export, detoxification

product: copper transporting ATPase

essential: no

synonyms: yvgX

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.1|Copper]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,441,121 → 3,443,529

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + Cu¹⁺(In) = ADP + phosphate + Cu¹⁺(Out) (according to Swiss-Prot)

Protein family

• Type IB subfamily (according to Swiss-Prot)

[SW|Domains]

• two N-terminal soluble domains, CopAa and CopAb, connected by a short linker

Structure

• [PDB|2RML] ( N-terminal soluble domain)
• [PDB|4BBJ] (the protein from ''Legionella pneumophila'', 45% identity, 79% similarity) [Pubmed|24317491]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-A490 (yvgX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/490 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Reviews

• 19824702,23046649

Original publications

• 19751213,11922674,12590580,14663075,12779235,12644235,18048925,14514665,14711369,19378562,15212800,20233928,24317491,22531974,22077885,28078344