SubtiBank SubtiBank
ykuQ
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ykuQ

similar to tetrahydrodipicolinate succinylase
locus
BSU14180
pI
4.68
mw
24.84 kDa
protein length
236 aa Sequence Blast
gene length
708 bp Sequence Blast
function
biosynthesis of lysine and peptidoglycan
product
similar to tetrahydrodipicolinate succinylase
essential
yes
ec
2.3.1.89
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.7|Biosynthesis/ acquisition of lysine/ threonine/ based on similarity]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,488,973 → 1,489,683

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + L-2-acetamido-6-oxoheptanedioate (according to Swiss-Prot)
  • Protein family

  • transferase hexapeptide repeat family (according to Swiss-Prot)
  • Structure

  • [PDB|3R8Y] (from ''Bacillus anthracis'', 80% identity, 96% similarity)
  • Expression and Regulation

    Operons

    genes
    [gene|716FE06124544C05CC34BA2E643B4DBD27F04998|ykuQ]-[gene|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|ykuR]-[gene|3E7E0342AA86AFCC95F5CD5BEA5B10166DCE2721|ykuS]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B340 (ykuQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1339 NBRP B. subtilis, Japan]