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yvrHb [2019-02-01 14:16:35]
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yvrHb [2019-02-01 14:16:35]

two-component response regulator (OmpR family)
locus
BSU33221
pI
5.24
mw
27.00 kDa
protein length
237 aa Sequence Blast
gene length
714 bp Sequence Blast
function
regulation cell surface maintenance
product
two-component response regulator (OmpR family)
essential
no
synonyms
yvrH
Outlinks
KEGGBsubCycUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    3,408,353 3,409,066

    Phenotypes of a mutant

  • unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin) [Pubmed|16306698]

    • The protein

    Catalyzed reaction/ biological activity

  • transcription activator [Pubmed|16306698]

    • Protein family

  • [SW|OmpR family] of two-component response regulators

    • Modification

  • phosphorylation (Asp) by [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG] [Pubmed|16306698]

    • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity

    • Structure

  • [PDB|2OQR] (from Mycobacterium tuberculosis, 37% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]-[gene|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG]-[gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B050 (yvrH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1049 NBRP B. subtilis, Japan]
  • BKE33221 ([gene|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33221 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG, downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG
  • BKK33221 ([gene|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33221 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG, downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG

    • References

  • 10094672,18820022,16306698