hemB

porphobilinogen synthase

locus: BSU_28130

pI: 4.98

mw: 36.05 kDa

protein length: 324 aa

gene length: 975 bp

function: heme biosynthesis

product: porphobilinogen synthase

essential: no

ec: 4.2.1.24

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,874,202 2,875,176

The protein

Catalyzed reaction/ biological activity

• 2 5-aminolevulinate --> porphobilinogen + 2 H₂O (according to UniProt)

Protein family

• ALAD family (single member, according to UniProt)

Modification

• phosphorylated on Arg-217 [Pubmed|22517742]

[SW|Cofactors]

• zinc

Structure

• [PDB|1W5Q] (from ''Pseudomonas aeruginosa'', 48% identity) [Pubmed|15644204]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG
• BKK28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG

References

Reviews

• 28123057

Original Publications

• 10217486,11532148,1672867,4214010,804803,12926268,20823524,22517742,15644204