thyA

thymidylate synthase A

locus: BSU_17680

pI: 5.50

mw: 32.65 kDa

protein length: 279 aa

gene length: 840 bp

function: biosynthesis of thymidine nucleotides

product: thymidylate synthase A

essential: no

ec: 2.1.1.45

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]

Gene

Coordinates: 1,902,219 1,903,058

The protein

Catalyzed reaction/ biological activity

• (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)

Protein family

• thymidylate synthase family (with [protein|9F9643D51E9ABA537892B19312491697CECDDCCF|ThyB], according to UniProt)

Paralogous protein(s)

• [protein|9F9643D51E9ABA537892B19312491697CECDDCCF|ThyB]

Structure

• [PDB|1B02] [pubmed|10091656]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE17680 ([gene|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTATTCATATCCTTC, downstream forward: _UP4_TAATGCTGCCTTTTTATTGT
• BKK17680 ([gene|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTATTCATATCCTTC, downstream forward: _UP4_TAATGCTGCCTTTTTATTGT

References

Reviews

• 7574499

Original publications

• 418407,11267663,28700593,10091656,7704257,9648749,111614,29150519