asnB

asparagine synthase (glutamine-hydrolysing)

locus: BSU_30540

pI: 5.61

mw: 72.49 kDa

protein length: 632 aa

gene length: 1899 bp

function: control of peptidoglycan hydrolysis

product: asparagine synthase (glutamine-hydrolysing))

essential: no

ec: 6.3.5.4

synonyms: asn

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine]

Gene

Coordinates: 3,125,777 3,127,675

Phenotypes of a mutant

• the mutant requires excess Mg²⁺ for growth [pubmed|28317238]

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)
• amidation of meso-diaminopimelic (mDAP) acid in peptidoglycan [pubmed|28317238]

Protein family

• asparagine synthetase family (with [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|AsnO] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|AsnH], according to UniProt)

[SW|Domains]

• [SW|Glutamine amidotransferase type-2 domain] (aa 2-214) (according to UniProt)

Structure

• [PDB|1CT9] (from ''E. coli'', 29% identity) [Pubmed|10587437]

Expression and Regulation

Operons

• genes: [gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]
  description: [Pubmed|10498721]

  regulation

  • constitutive

Biological materials

Mutant

• MGNA-A141 (asnB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/141 NBRP B. subtilis, Japan]

References

Reviews

• 12859215,11395405

Original publications

• 10498721,10587437,28317238