SubtiBank SubtiBank
asnB
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asnB

asparagine synthase (glutamine-hydrolysing)
locus
BSU_30540
pI
5.61
mw
72.49 kDa
protein length
632 aa Sequence Blast
gene length
1899 bp Sequence Blast
function
control of peptidoglycan hydrolysis
product
asparagine synthase (glutamine-hydrolysing))
essential
no
ec
6.3.5.4
synonyms
asn

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine]
  • Gene

    Coordinates
    3,125,777 3,127,675

    Phenotypes of a mutant

  • the mutant requires excess Mg2+ for growth [pubmed|28317238]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)
  • amidation of meso-diaminopimelic (mDAP) acid in peptidoglycan [pubmed|28317238]
  • Protein family

  • asparagine synthetase family (with [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|AsnO] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|AsnH], according to UniProt)
  • Structure

  • [PDB|1CT9] (from ''E. coli'', 29% identity) [Pubmed|10587437]
  • Expression and Regulation

    Operons

    genes
    [gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]
    description
    [Pubmed|10498721]

    regulation

  • constitutive
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    Biological materials

    Mutant

  • MGNA-A141 (asnB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/141 NBRP B. subtilis, Japan]
  • References

    Reviews

  • 12859215,11395405
  • Original publications

  • 10498721,10587437,28317238