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ldt [2018-02-13T16:06:28.000Z]
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ldt [2018-02-13T16:06:28.000Z]

L,D-transpeptidase involved in cell wall synthesis
locus
BSU14040
pI
10.25
mw
17.59 kDa
protein length
164 aa Sequence Blast
gene length
492 bp Sequence Blast
function
cell wall biosynthesis
product
L,D-transpeptidase
essential
no
synonyms
ykuD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

    • Gene

    Coordinates
    1,476,518 → 1,477,012

    The protein

    Paralogous protein(s)

  • [protein|293794B65B9EEB7F049138F73893303BABFB9A2A|YqjB]

    • [SW|Domains]

  • contains a N-terminal N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]

    • Structure

  • [PDB|1Y7M] [Pubmed|16287140]
  • [PDB|2MTZ] (complex with a peptidoglycan hexamuropeptide) [Pubmed|25429710]

    • [SW|Localization]

  • spore wall (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]
    description
    [Pubmed|11011148]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|11011148], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|11011148]
    • view in new tab

    genes
    [gene|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE]-[gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]-[gene|DDAE68CD8D7F0D9C46E62D0EDBF4631C9EC4C236|ykuC]
    description
    [Pubmed|22383849]

    regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|11011148]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A766 (ykuD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/766 NBRP B. subtilis, Japan]
  • BKE14040 (Δ[gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGTTTCCTCATCCTCCTTT, downstream forward: _UP4_TAATGTTTTCTTATTTGTTT
  • BKK14040 (Δ[gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGTTTCCTCATCCTCCTTT, downstream forward: _UP4_TAATGTTTTCTTATTTGTTT

    • References

    Reviews

  • 18430080

    • Original publications

  • 12850135,11011148,17311917,22383849,22278298,22579252,16287140,25429710