ytkP

similar to cysteine synthase

locus: BSU_29970

pI: 4.93

mw: 32.91 kDa

protein length: 311 aa

gene length: 933 bp

function: unknown

product: unknown

essential: no

ec: 2.5.1.47

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,066,451 → 3,067,386

The protein

Catalyzed reaction/ biological activity

• O(3)-acetyl-L-serine + H₂S --> L-cysteine + acetate (according to UniProt)

Protein family

• Cysteine synthase/cystathionine beta-synthase family (with [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|CysK] and [protein|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|MccA], according to UniProt)

Paralogous protein(s)

• [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|CysK], [protein|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|MccA]

[SW|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|2EGU] (from Geobacillus Kaustophilus 57% identity)

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B534 (ytkP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1533 NBRP B. subtilis, Japan]
• BKE29970 (Δ[gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA, downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA
• BKK29970 (Δ[gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA, downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA