czcO

similar to flavin-containing monooxygenase, facilitates cation export via [protein|7FB5BCC16D688465A820F436F657C49625257884|CzcD]

locus: BSU_26640

pI: 9.83

mw: 38.91 kDa

protein length: 345 aa

gene length: 1038 bp

function: resistance to toxic metal ions

product: unknown

essential: no

synonyms: trkA,yrdP

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.4|Trace metals/ Other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]

Gene

Coordinates: 2,722,767 2,723,804

The protein

Structure

• [PDB|4USQ] (from Cellvibrio sp., 23% identity) [pubmed|25383040]

Expression and Regulation

Operons

• genes: [gene|7FB5BCC16D688465A820F436F657C49625257884|czcD]-[gene|6D6C324675631B5CAB165D6DF72AA45BF3DB6F29|czcO]
  description: [Pubmed|15948947]

  regulatory mechanism

  • [protein|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|CzrA]: repression, [Pubmed|15948947], in [regulon|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|CzrA regulon]

  regulation

  • induced in the presence of toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) ([protein|search|CzrA]) [Pubmed|15948947]

Biological materials

Mutant

• BKE26640 ([gene|6D6C324675631B5CAB165D6DF72AA45BF3DB6F29|czcO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTATCACCCCGCCAAT, downstream forward: _UP4_TAAACGAAGCAGAGCAGTAA
• BKK26640 ([gene|6D6C324675631B5CAB165D6DF72AA45BF3DB6F29|czcO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTATCACCCCGCCAAT, downstream forward: _UP4_TAAACGAAGCAGAGCAGTAA

References

• 15948947,12100555,25383040