tsaB [Fri Jan 29 2016 18:07:50 GMT+0100 (CET)]

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

locus: BSU05920

pI: 5.00

mw: 25.00 kDa

function: tRNA modification

product: tRNA modification enzyme

essential: yes

synonyms: ydiC

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|essential genes], [SW|translation]

Gene

Coordinates on the chromosome (coding sequence): 642,111 -> 642,800

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|search|TsaB]-[protein|search|TsaD]-[protein|search|TsaE]) [Pubmed|23072323,21183954]

Structure

• [PDB|2A6A] (from ''Thermotoga maritima'', 24% identity)

[SW|Interactions]

• [protein|search|TsaB]-[protein|search|TsaD] [Pubmed|23072323,21285948]
• [protein|search|TsaB]-[protein|search|TsaE] [Pubmed|23072323]

Expression and Regulation

Operon

• ''[protein|search|thiL]-[protein|search|tsaE]-[protein|search|tsaB]-[protein|search|ydiD]-[protein|search|tsaD]'' [Pubmed|22383849]

References

• 19376873,17005971,21285948,23072323,22383849