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tsaB

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

locus: BSU05920

pI: 5.76

mw: 25.10 kDa

protein length: 229 aa

gene length: 690 bp

function: tRNA modification

product: tRNA modification enzyme

essential: yes

synonyms: ydiC

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 642,111 642,800

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]

Protein family

• KAE1 / TsaD family (with [protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD], according to UniProt)

Structure

• [PDB|2A6A] (from ''Thermotoga maritima'', 24% identity) [Pubmed|20944216]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
  description: [Pubmed|22383849]

References

• 19376873,17005971,21285948,23072323,22383849,20944216,29741707