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fliW [2019-05-24 09:53:15]
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fliW [2019-05-24 09:53:15]

checkpoint protein for hag expression, CsrA anatagonist
locus
BSU35380
pI
4.34
mw
16.03 kDa
protein length
143 aa Sequence Blast
gene length
432 bp Sequence Blast
function
control of CsrA activity
product
flagellar assembly factor, CsrA anatagonist
essential
no
synonyms
yviF

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]
  • Gene

    Coordinates
    3,636,264 3,636,695

    The protein

    Catalyzed reaction/ biological activity

  • [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] binds [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] when [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|Hag] is secreted after hook completion (and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] released from the [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|Hag]-[protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] complex) to allow translation of the'' [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mRNA [Pubmed|21895793]
  • Protein family

  • fliW family (according to Swiss-Prot)
  • Structure

  • [PDB|5DMD] (from ''Geobacillus thermodenitrificans'') [Pubmed|27551070]
  • [PDB|5DMB] ([protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW]-[protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] complex) [Pubmed|27551070]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] form a conserved module in many bacteria [Pubmed|21895793,27516547]
  • Expression and Regulation

    Operons

    genes
    [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412657], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8045879], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8412657], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|21736639], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19898538], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
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    Biological materials

    Mutant

  • MGNA-A339 (yviF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/339 NBRP B. subtilis, Japan]
  • BKE35380 ([gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCACGATCCTTTTC, downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
  • BKK35380 ([gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCACGATCCTTTTC, downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
  • References

    Reviews

  • 22672726,25251856
  • Original Publications

  • 16936039,23144244,21895793,26577401,27516547,27551070