SubtiBank SubtiBank
hisS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

hisS

histidyl-tRNA synthetase
locus
BSU27560
pI
5.23
mw
47.97 kDa
protein length
424 aa Sequence Blast
gene length
1272 bp Sequence Blast
function
translation
product
histidyl-tRNA synthetase
essential
yes
ec
6.1.1.21
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,816,535 → 2,817,809

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)
  • Protein family

  • class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|1H4V] (from ''Thermus thermophilus'', 43% identity, 61% similarity) [Pubmed|11399074]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|69C7EF7E76821FF3D9E866838B24C3866CA9C189|hisS]-[gene|2B61D17950ADE0247B0CF4AE33548E15C0DDCD9B|aspS]

    regulatory mechanism

  • [regulon|T-box|T-box]: transcriptional antitermination, via tRNA controlled [SW|RNA switch], in [regulon|T-box|T-box]
  • regulation

  • induced by histidine or aspartate limitation ([protein|search|T-box]) [Pubmed|19258532]
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    References

  • 19258532