rocA

3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

Locus: BSU_37780

Isoelectric point: 5.58

Molecular weight: 56.15 kDa

Protein length: 515 aa

Gene length: 1548 bp

Function: arginine, ornithine and citrulline utilization

Product: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

Essential: no

E.C.: 1.5.1.12

Synonyms: ipa-76d

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of arginine/ ornithine
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 3,878,966 3,880,513

The protein

Catalyzed reaction/ biological activity

• H₂O + L-glutamate 5-semialdehyde + NAD⁺ --> 2 H⁺ + L-glutamate + NADH (according to UniProt)

Protein family

• aldehyde dehydrogenase family (according to UniProt)

Paralogous protein(s)

• IolA, YwdH, GabD, YfmT, AldX, AldY, GbsA, PutC, YcbD, DhaS

Modification

• phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed

Structure

• 3RJL (1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis, 68% identity, 88% similarity)

Expression and Regulation

Operons

• Genes: rocA-rocB-rocC
  Description: PubMed

  Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • RocR: activation, PubMed, in RocR regulon
  • AhrC: activation, PubMed, in AhrC regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

  Additional information

  • expression of the rocA-rocB-rocC operon is increased upon depletion of nusA (resulting from increased ahrC expression) Reference

Biological materials

Mutant

• BKE37780 (rocA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG, downstream forward: _UP4_TGATTTGAGAGAAATAAAAT
• BKK37780 (rocA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG, downstream forward: _UP4_TGATTTGAGAGAAATAAAAT

References

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