tagT

phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

locus: BSU_35840

pI: 9.55

mw: 35.65 kDa

protein length: 322 aa

gene length: 969 bp

function: transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan

product: phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan

essential: no

synonyms: ywtF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.6|Export of anionic polymers and attachment to peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,694,239 3,695,207

Phenotypes of a mutant

• a ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]'' mutant has no phenoype, the triple ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT] [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant is unable to grow under normal conditions [Pubmed|21964069]

The protein

Protein family

• LytR/CpsA/Psr (LCP) family (with [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|TagU] and [protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|TagV], according to UniProt) [Pubmed|19099556]

Paralogous protein(s)

• [protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|TagV], [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|TagU]

[SW|Cofactors]

• Mg²⁺ [Pubmed|29107701,21964069]

Structure

• [PDB|6UF5] (aa 46-322) [pubmed|31969390]
• [PDB|3MEJ]
• [PDB|4DE9] [Pubmed|22432711]
• [PDB|6MPS] (bound to LIIa-WTA] [Pubmed|29402087]
• [PDB|6MPT] (bound to LI-WTA] [Pubmed|29402087]

[SW|Localization]

• cytoplasmic membrane (with extracytoplasmic catalaytic domain) [Pubmed|21964069]
• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]) [pubmed|18179421]

• genes: [gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]-[gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]) [pubmed|18179421]

Biological materials

Mutant

• MGNA-A547 (ywtF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/547 NBRP B. subtilis, Japan]
• BKE35840 ([gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATCAATACCTCACGT, downstream forward: _UP4_TAATGAACGGGATGGCGATT
• BKK35840 ([gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATCAATACCTCACGT, downstream forward: _UP4_TAATGAACGGGATGGCGATT

References

Reviews

• 24024634

Original publications

• 18957862,9353933,18179421,19099556,21964069,22432711,29107701,29402087,31969390