SubtiBank SubtiBank
eno [Mon Oct 10 2016 11:03:12 GMT+0200 (CEST)]
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eno [Mon Oct 10 2016 11:03:12 GMT+0200 (CEST)]

enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein
locus
BSU33900
pI
4.00
mw
46.00 kDa
function
enzyme in glycolysis/ gluconeogenesis
product
enolase
essential
Yes
ec
4.2.1.11
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.5|Effectors of RNA degradation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|essential genes], [SW|membrane proteins], [SW|phosphoproteins], [SW|universally conserved proteins], [SW|most abundant proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CggR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,476,555 -> 3,477,847

    Phenotypes of a mutant

  • no growth on LB, requires glucose and malate [Pubmed|23420519]
  • essential according to Kobayashi et al. on LB [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-phospho-D-glycerate = phosphoenolpyruvate H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate H(2)O
  • Protein family

  • enolase family (according to Swiss-Prot)
  • Kinetic information

  • reversible Michaelis-Menten [Pubmed|25885]
  • [SW|Domains]

  • substrate binding domain (366–369)
  • Modification

  • phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [Pubmed|17218307,16493705,17726680]
  • [SW|Cofactors]

  • Mg2
  • Effectors of protein activity

  • Inhibited by EDTA [Pubmed|25885]
  • Structure

  • [PDB|4A3R] [Pubmed|22198292]
  • [PDB|1W6T] (from ''Streptococcus pneumoniae'') [Pubmed|15476816]
  • [SW|Localization]

  • cytoplasm [Pubmed|16479537,27708634]
  • membrane associated [Pubmed|18763711]
  • exported, this requires a long, unbent α-helix (from A108 to L126) [Pubmed|15003462,21856851]
  • [SW|Interactions]

  • forms octamers [Pubmed|22198292]
  • part of the [SW|RNA degradosome] [Pubmed|19193632]
  • [protein|search|Eno]-[protein|search|PfkA] [Pubmed|19193632], K(D) of the interaction: 40 nM [Pubmed|22198292]
  • [protein|search|Eno]-[protein|search|Rny] [Pubmed|19193632,21803996], K(D) of the interaction: 100 nM [Pubmed|22198292]
  • [protein|search|Eno]-[protein|search|CshA] [Pubmed|20572937]
  • Additional information

  • Enolase is a [SW|moonlighting protein]. [Pubmed|19193632]
  • There are indications that this enzyme is an octamer [Pubmed|25885]
  • [SW|universally conserved protein]
  • extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]
  • Expression and Regulation

    Operon

  • ''[protein|search|cggR]-[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • ''[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11489127]
  • Regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • ''[protein|search|cggR]'': induced by glycolytic substrates [protein|search|CggR] [Pubmed|11489127]
  • ''[protein|search|pgk]'': constitutive [Pubmed|11489127]
  • Regulatory mechanism

  • transcription repression by [protein|search|CggR] [Pubmed|11489127]
  • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Biological materials

    Mutant

  • GP594 (''eno''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP599 (''eno''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP698 (''eno''-''[protein|search|pgm]''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • Expression vector

  • pGP1426 (expression of ''[protein|search|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1500 (expression of ''[protein|search|pgm]'' and ''[protein|search|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP563 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • see ''[protein|search|pgk]
  • GFP fusion

  • pHT315-yfp-eno, available in [SW|Mijakovic] lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1214 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • References

    Reviews

  • 8994873
  • Subcellular localization of enolase

  • 16479537,18763711,20497499,15003462,21856851,24642254,27708634
  • Other original publications

  • 23420519,17726680,17218307,12850135,19193632,11489127,8021172,17505547,25885,20572937,15476816,9988532,21803996,22198292,15378759