SubtiBank SubtiBank
eno
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eno

enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein
locus
BSU33900
pI
4.49
mw
46.42 kDa
protein length
430 aa Sequence Blast
gene length
1290 bp Sequence Blast
function
enzyme in glycolysis/ gluconeogenesis
product
enolase
essential
Yes
ec
4.2.1.11
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    3,476,555 → 3,477,847

    Phenotypes of a mutant

  • no growth on LB, requires glucose and malate [Pubmed|23420519]
  • essential according to Kobayashi et al. on LB [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-phospho-D-glycerate = phosphoenolpyruvate H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate H(2)O
  • Protein family

  • enolase family (according to Swiss-Prot)
  • Kinetic information

  • reversible Michaelis-Menten [Pubmed|25885]
  • [SW|Domains]

  • substrate binding domain (366–369)
  • Modification

  • phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [Pubmed|17218307,16493705,17726680]
  • [SW|Cofactors]

  • Mg2
  • Effectors of protein activity

  • Inhibited by EDTA [Pubmed|25885]
  • Structure

  • [PDB|4A3R] [Pubmed|22198292]
  • [PDB|1W6T] (from ''Streptococcus pneumoniae'') [Pubmed|15476816]
  • [SW|Localization]

  • cytoplasm [Pubmed|16479537,27708634]
  • membrane associated [Pubmed|18763711]
  • exported, this requires a long, unbent α-helix (from A108 to L126) [Pubmed|15003462,21856851]
  • Additional information

  • Enolase is a [SW|moonlighting protein]. [Pubmed|19193632]
  • There are indications that this enzyme is an octamer [Pubmed|25885]
  • [SW|universally conserved protein]
  • extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
    description
    [Pubmed|11489127]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489127], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]: repression, [Pubmed|11489127], in [regulon|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR regulon]
  • regulation

  • ''[protein|search|cggR]'': induced by glycolytic substrates [protein|search|CggR] [Pubmed|11489127]
  • view in new tab

    genes
    [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
    description
    [Pubmed|11489127]
    view in new tab

    Biological materials

    Mutant

  • GP594 (Δ''eno''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP599 (Δ''eno''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP698 (Δ''eno''-''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • Expression vector

  • pGP1426 (expression of ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1500 (expression of ''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]'' and ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP563 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
  • pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
  • GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • see [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]
  • GFP fusion

  • pHT315-yfp-eno, available in [SW|Mijakovic] lab
  • GP1700 (in [SW|pBP43]), expression of '' eno-GFP''::''spc'' under the native promoter, available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
  • FLAG-tag construct

  • GP1214 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|21803996]
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • References

    Reviews

  • 8994873
  • Subcellular localization of enolase

  • 16479537,18763711,20497499,15003462,21856851,24642254,27708634
  • Other original publications

  • 23420519,17726680,17218307,12850135,19193632,11489127,8021172,17505547,25885,20572937,15476816,9988532,21803996,22198292,15378759,28516784