ssuB

aliphatic sulfonate [SW|ABC transporter] (binding protein)

locus: BSU_08830

pI: 8.67

mw: 30.08 kDa

protein length: 255 aa

gene length: 768 bp

function: sulfonate uptake

product: aliphatic sulfonate [SW|ABC transporter] (binding protein)

essential: no

synonyms: ygaL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 961,394 962,161

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|3DC6A032B0F076A460FE2C3244D747CFC7583FE7|YtlC]

[SW|Domains]

• [SW|ABC transporter domain] (aa 7-231) (according to UniProt)

Structure

• [PDB|1Q1B] (MalK from E. coli, 38% identity) [pubmed|14527411]

[SW|Localization]

• associated to the membrane (via [protein|91EAC386AD3A373E7CDE0265AA23786ADC55558A|SsuC]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]
  description: [Pubmed|11251850]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11251850], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

Biological materials

Mutant

• MGNA-A249 (ygaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/249 NBRP B. subtilis, Japan]
• BKE08830 ([gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG
• BKK08830 ([gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG

References

• 15937167,11251850,10092453,16513748,9782504,14527411