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satA [2019-01-20 17:16:43]
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satA [2019-01-20 17:16:43]

streptothricine acetyl-transferase
locus
BSU40740
pI
5.83
mw
20.25 kDa
protein length
173 aa Sequence Blast
gene length
519 bp Sequence Blast
function
resistance to streptothricine
product
streptothricine acetyl-transferase
essential
no
synonyms
yyaR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

    • Gene

    Coordinates
    4,185,160 → 4,185,681

    Phenotypes of a mutant

  • sensitive to streptothricine [pubmed|28842538]

    • The protein

    Catalyzed reaction/ biological activity

  • acetylation of streptothricin at the expense of acetyl-CoA [pubmed|28842538]

    • Kinetic information

  • Km (streptothricine): 1 µM [pubmed|28842538]
  • Km (acetyl-CoA): 107 µM [pubmed|28842538]

    • Structure

  • [PDB|3PP9] (complex with acetyl-CoA, from ''B. anthracis'', 35% identity, 57% similarity)

    • Biological materials

    Mutant

  • MGNA-B856 (yyaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1855 NBRP B. subtilis, Japan]
  • BKE40740 (Δ[gene|66D38B4C38D6C7299502F2BD5C06102A2FF14F92|satA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA
  • BKK40740 (Δ[gene|66D38B4C38D6C7299502F2BD5C06102A2FF14F92|satA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA

    • References

    Research papers

  • 28842538