SubtiBank SubtiBank
citA [Mon Aug 31 2015 13:53:21 GMT+0200 (CEST)]
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citA [Mon Aug 31 2015 13:53:21 GMT+0200 (CEST)]

minor citrate synthase
locus
BSU09440
pI
5.78
mw
40.00 kDa
function
unknown
product
minor citrate synthase
essential
no
ec
2.3.3.1
synonyms
citA1

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • syns
    citA1

    [SW|Categories] containing this gene/protein

  • [SW|poorly characterized/ putative enzymes], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CitR regulon]
  • The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
  • Protein family

  • citrate synthase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|MmgD], [protein|search|CitZ]
  • Structure

  • [http://www.proteopedia.org/wiki/index.php/2c6x 2C6X]
  • Expression and Regulation

    Operon

  • ''[protein|search|citA]-[protein|search|yhdF]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8045898]
  • Regulatory mechanism

  • [protein|search|CitR]: transcription repression [Pubmed|8045899]
  • Biological materials

    Mutant

  • GP676 (erm), available in [SW|Jörg Stülke]'s lab
  • GP1282 (''[protein|search|citA]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP1753 (''[protein|search|citR] [protein|search|citA]''::''aphA3''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP2516 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1287 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

  • 8045899,8045898,22383849