licB

lichenan-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIB of the [category|SW 1.2.2|PTS], [category|SW 3.4.3|Trigger enzyme], control of [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR] activity

locus: BSU_38590

pI: 6.31

mw: 10.82 kDa

protein length: 102 aa

gene length: 309 bp

function: lichenan uptake and phosphorylation, control of LicR activity

product: lichenan-specific [category|SW 1.2.2|PTS], EIIB component

essential: no

ec: 2.7.1.69

synonyms: celA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,961,566 3,961,874

The protein

Protein family

• [category|SW 1.2.2|PTS] permease, lactose family [Pubmed|10627040]

Paralogous protein(s)

• [protein|00A4A90685CED4AD46829C45718CF26F1D62F5D3|GmuB]

[SW|Domains]

• [SW|PTS EIIB domain] type-3 (aa 1-102) (according to UniProt)

Modification

• phosphorylation on Ser-37 [Pubmed|17218307]

Structure

• [PDB|1e2b] (from ''E. coli'', 40% identity) [Pubmed|9041631]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE38590 ([gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGACCCCCTAGA, downstream forward: _UP4_GTGTCATAGAGAGGGGCGGG
• BKK38590 ([gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGACCCCCTAGA, downstream forward: _UP4_GTGTCATAGAGAGGGGCGGG

References

• 19087206,8990303,16872404,10438772,10627040,17218307,8990303,9041631