SubtiBank SubtiBank
dnaE [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

dnaE [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

DNA polymerase III (alpha subunit), part of the replisome
locus
BSU29230
pI
6.02
mw
125.00 kDa
function
DNA replication
product
DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|LexA regulon]
  • Gene

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Protein family

  • DnaE subfamily (according to Swiss-Prot)
  • Structure

  • [PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]
  • [SW|Localization]

  • forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|search|MutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
  • cytoplasm (according to Swiss-Prot)
  • [SW|Interactions]

  • part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
  • [protein|search|DnaE]-[protein|search|MutL] [Pubmed|21958350]
  • [protein|search|DnaE]-[protein|search|MutS] [Pubmed|21958350]
  • [protein|search|DnaE]-[protein|search|DnaN] [Pubmed|21958350]
  • [protein|search|DnaE]-[protein|search|SsbA] [Pubmed|21958350]
  • [protein|search|DnaE]-[protein|search|DnaC] [Pubmed|23563155]
  • [protein|search|DnaE]-[protein|search|DnaG] [Pubmed|23563155]
  • [protein|search|DnaE]-[protein|search|HolA] [Pubmed|23563155]
  • [protein|search|DnaE]-[protein|search|PolC] [Pubmed|23563155]
  • Expression and Regulation

    Operon

  • ''[protein|search|dnaE]'' (according to [http://dbtbs.hgc.jp/COG/prom/dnaE.html DBTBS])
  • Regulation

  • induced by DNA damage ([protein|search|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
  • Regulatory mechanism

  • [protein|search|LexA]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
  • References

    Reviews

  • 22933559
  • Original publications

  • 11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598