SubtiBank SubtiBank
dnaE
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

dnaE

lagging strand DNA polymerase III (alpha subunit), part of the [SW|replisome]
locus
BSU29230
pI
6.02
mw
125.14 kDa
protein length
1115 aa Sequence Blast
gene length
3345 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication](lagging strand)
product
lagging strand DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,991,269 → 2,994,616

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Protein family

  • DnaE subfamily (according to Swiss-Prot)
  • Structure

  • [PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]
  • [SW|Localization]

  • forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|14593098,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
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    References

    Reviews

  • 22933559,28073967
  • Original publications

  • 11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598,28575448,28878042