yesW

rhamnogalacturonan lyase, generates oligosaccharides

locus: BSU_07050

pI: 5.97

mw: 67.41 kDa

protein length: 620 aa

gene length: 1863 bp

function: utilization of rhamnogalacturonan

product: rhamnogalacturonan lyase, generates oligosaccharides

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

Gene

Coordinates: 770,234 772,096

The protein

Catalyzed reaction/ biological activity

• Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end (according to UniPort)

Protein family

• polysaccharide lyase 11 family (with [protein|217BAA94FB926CDA7CA8800BC6B08371792CEE31|YesX], according to UniProt)

Paralogous protein(s)

• [protein|217BAA94FB926CDA7CA8800BC6B08371792CEE31|YesX]

[SW|Domains]

• Rhamnose is bound near the active site, but there are also additional rhamnose-binding sites far away from the active site, which possibly function as a carbohydrate-binding region (according to UniProt)

Structure

• [PDB|2Z8R]

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
  description: [Pubmed|19651770]

  regulatory mechanism

  • [protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR]: activation, [Pubmed|19651770], in [regulon|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR regulon]

  regulation

  • induced by pectin ([protein|search|RhgR]) [Pubmed|19651770]

Biological materials

Mutant

• BKE07050 ([gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTGTAAGCGCTTA, downstream forward: _UP4_TAACTGAAAGGGGGAAGGAA
• BKK07050 ([gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTGTAAGCGCTTA, downstream forward: _UP4_TAACTGAAAGGGGGAAGGAA

References

Reviews

• 26255130

Original publications

• 19651770,17449691,17947240,19193638,16682770