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tlrB
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tlrB

23S rRNA (guanine-N(1)-)-methyltransferase, confers resistance against the macrolide antibiotic tylosin (produced by Streptomyces fradiae)
locus
BSU_39010
pI
8.13
mw
31.32 kDa
protein length
282 aa Sequence Blast
gene length
849 bp Sequence Blast
function
resistance against tylosin
product
23S rRNA (guanine-N(1)-)-methyltransferase
essential
no
synonyms
yxjB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

    • Gene

    Coordinates
    4,004,288 4,005,136

    The protein

    Catalyzed reaction/ biological activity

  • methylates G748 of 23S rRNA in the peptide exit tunnel [pubmed|31719185]

    • Protein family

  • [SW|Methyltransferase superfamily] (according to UniProt)

    • Structure

  • [PDB|1P91] (from E. coli, 31% identity) [pubmed|14999102]

    • Expression and Regulation

    Operons

    genes
    [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]
    description
    [Pubmed|10746760]

    regulatory mechanism

  • [protein|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL]: attenuation, [pubmed|31719185], in [regulon|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL regulon]

    • regulation

  • induced in the presence of tylosin (transcription attenuation and translation attenuation in the absence of tylosin) [Pubmed|31719185]
  • induction depends on [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-dependent [SW|RNA polymerase] pausing between the leader peptide [protein|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL] and [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB] coding sequences [Pubmed|31719185]

    • additional information

  • a [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|NusA]-dependent terminator and an SD-sequestering hairpin are present in the [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB] leader [Pubmed|31719185,27120414]
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    Biological materials

    Mutant

  • MGNA-B726 (yxjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1725 NBRP B. subtilis, Japan]
  • 1A995 ( ''yxjB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A995&Search=1A995 BGSC]
  • BKE39010 ([gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC
  • BKK39010 ([gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

    • References

  • 10746760,27120414,14999102,31719185